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1. Construction of antisense cDNA library
2. Plant transformation
3. Selection of transgenic plants
4. Screening of mutants
5. Isolation of insert cDNA
6. Analysis of sequencing data for insert cDNA
7. Plant retransformation


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Çʼö À¯ÀüÀÚÀÇ ±â´É¼º µ¿Á¤¿¡ ´Ù¸¥ ±â´É¼º À¯Àüü ¿¬±¸º¸´Ù ¸¹Àº ÀåÁ¡À» °¡Áø´Ù.
º¯ÀÌüÀÇ Ç¥ÇöÇüÀÌ ¿ì¼ºÀ¸·Î ¹ß»ý Ãʱ⿡ ½±°Ô º¯ÀÌü¸¦ µ¿Á¤ÇÒ ¼ö ÀÖ´Ù.
cf. insertional mutagenesis´Â º¯ÀÌüÀÇ Ç¥ÇöÇüÀÌ ¿­¼ºÀ¸·Î ³ªÅ¸³².
Multigene family¿¡¼­µµ in vivo »óÅ·ΠƯÁ¤ À¯ÀüÀÚÀÇ ±â´ÉÀ» µ¿Á¤ÇÒ ¼ö ÀÖ´Ù.
Çʼö À¯ÀüÀÚÀÇ antisense disruptionÀº º¯ÀÌüÀÇ È¸º¹µÇÁö ¾Ê´Â Ä¡»ç¸¦ À̲ø °ÍÀÌ´Ù. ±×·¯¹Ç·Î inducible vectorÀÇ »ç¿ëÀÌ ÇʼöÀûÀÌ´Ù.
Simple PCR method¸¦ ÅëÇÏ¿© ½±°Ô À¯ÀüÀÚ¸¦ cloneÇÒ ¼ö ÀÖ´Ù.



 
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